Summary: Accurate spatiotemporal control of multicellular self-organization by various signaling pathways is essential for developmental stages.In particular, evolutionarily conserved Wnt signaling serves as a major morphogenetic switch to determine the anteroposterior axis of vibrating table for chocolate the embryo.Here, we developed a genetically encoded optochemogenetic Wnt switch, named optochemoWnt, by coupling a blue light-inducible CRY2olig and rapamycin-inducible LRP6c clustering.The rationally designed optochemoWnt successfully modulated Wnt signaling with AND-gated patterns and demonstrated an improved signal-to-noise ratio (SNR).
The dual-triggered switch provides a safeguard to prevent signal leakage resulting from ambient canine spectra kc 3 intranasal single dose light sources under general laboratory conditions.OptochemoWnt expands the molecular toolbox available for the fields of developmental biology and tissue engineering.In addition, the AND-gated strategy of optochemoWnt may be used for other biomedical applications that integrate user defined switch elements with Boolean logic gates.